Cell Signaling Technology Myc-Tag (9B11) Mouse Monoclonal Antibody #2276

Product Overview

The Cell Signaling Technology Myc-Tag (9B11) Mouse Monoclonal Antibody #2276 is a premium, research-grade primary antibody specifically engineered for the detection of exogenously expressed proteins containing the Myc epitope tag. This antibody is widely recognized for its robust performance in identifying Myc tags fused to either the amino (N-terminal) or carboxy (C-terminal) terminus of recombinant proteins, providing researchers with exceptional versatility in protein expression studies.

This high-performance monoclonal antibody is raised against a synthetic peptide corresponding to residues 410-419 of the human c-Myc protein. The 9B11 clone ensures superior, consistent binding to the defined epitope across various experimental batches. Because of its high specificity, this reagent has become a standard tool in molecular biology laboratories worldwide for the precise detection, quantification, and analysis of Myc-tagged constructs in a vast range of diverse biological and experimental systems.

Key Features and Research Applications

• High Specificity: Engineered for precision, this antibody reliably targets the Myc epitope tag regardless of whether it is positioned at the N- or C-terminus of your protein of interest.
• Consistent Quality: As a monoclonal mouse antibody (clone 9B11), it provides unparalleled, lot-to-lot consistency, which is vital for reproducible data in longitudinal research projects.
• Broad Compatibility: Validated for a comprehensive suite of molecular applications, including Western blotting (WB), Simple Western (W-S), and immunoprecipitation (IP) workflows.
• Advanced Imaging: Highly effective for immunofluorescence (IF-IC) and flow cytometry (FC-FP), allowing for sensitive detection of tagged proteins within cellular contexts.
• Histology Ready: Extensively validated for immunohistochemistry on paraffin-embedded (IHC-P) tissue sections for spatial protein analysis.
• Chromatin Analysis: Fully compatible with chromatin immunoprecipitation (ChIP) experiments, enabling the identification of Myc-tagged transcription factors or regulatory elements.
• Reliable Source: Developed by Cell Signaling Technology, a leader in high-quality research antibodies, ensuring rigorous validation standards.

Technical Specifications

• Product Name: Myc-Tag (9B11) Mouse Monoclonal Antibody
• Catalog Number: #2276
• Clone Identifier: 9B11
• Host Species: Mouse (Monoclonal)
• Immunogen: Synthetic peptide corresponding to human c-Myc residues 410-419
• Validated Applications: Western Blot (W), Simple Western (W-S), Immunoprecipitation (IP), Immunohistochemistry - paraffin (IHC-P), Immunofluorescence (IF-IC), Flow Cytometry (FC-FP), Chromatin IP (ChIP)
• Reactivity: Cross-species detection of the Myc epitope tag sequence
• Storage Recommendations: Maintain activity by storing according to the provided datasheet; strictly avoid repeated freeze-thaw cycles
• Format: Research-grade primary antibody in buffered solution (reference the datasheet for exact concentrations)
• Brand: Distributed by Fractal Design with full manufacturer support

What's in the Box

• One primary vial containing the Myc-Tag (9B11) Mouse Monoclonal Antibody, catalog #2276.
• Comprehensive product datasheet including recommended starting dilutions, standardized protocol notes, and specific experimental tips.
• Detailed lot-specific quality control summary providing technical storage and handling guidelines.
• Supporting documentation, including certificates of analysis or QC summaries where applicable.

Integrate this antibody into your experimental workflows to ensure reliable, high-sensitivity detection of Myc-tagged protein expression. Before beginning your assays, always consult the included technical datasheet for recommended starting dilutions, optimal positive and negative controls, and sample preparation guidelines. For sensitive quantitative measurements or challenging experimental setups, utilize the optimized conditions provided by the manufacturer and perform internal validation to confirm performance within your specific laboratory environment and sample matrix.