Cell Signaling Technology Myc-Tag (9B11) Mouse Monoclonal Antibody #2276

Product Overview

The Myc-Tag (9B11) Mouse Monoclonal Antibody is a research-grade reagent developed by Cell Signaling Technology. This antibody recognizes the c-Myc epitope tag and is optimized for detection of exogenously expressed proteins that carry the Myc tag at either the amino or carboxy terminus. It provides high specificity and sensitivity across a range of common laboratory techniques, supporting reproducible protein analysis workflows. The antibody is suitable for routine expression verification as well as for more demanding applications such as immunoprecipitation and histological staining. Quality control and validation data support consistent performance in multiple experimental formats.

Key Features

• High specificity for Myc epitope tag fused to N- or C-terminus of target proteins
• Validated applications: Western Blot (W), Simple Western (W-S), Immunoprecipitation (IP)
• Supports Immunohistochemistry on paraffin sections (IHC-P) and Immunofluorescence (IF-IC)
• Compatible with Flow Cytometry protocols (FC-FP) for tagged protein detection
• Consistent lot-to-lot performance and extensive citation in peer-reviewed studies
• Suitable for quantitative and qualitative protein detection across sample types

Technical Specifications

• Host species: Mouse monoclonal
• Clone: 9B11
• Target: Myc epitope tag (c-Myc)
• Applications validated: WB, W-S, IP, IHC-P, IF-IC, FC-FP
• Immunogen: synthetic peptide corresponding to Myc tag
• Format: purified antibody suitable for direct use in standard protocols
• Storage conditions: store as recommended by manufacturer to preserve activity
• Quality control: tested for specificity and sensitivity in validated assays

What is in the Box

• Myc-Tag (9B11) Mouse Monoclonal Antibody vial(s) as specified on product label
• Certificate of analysis and lot-specific validation information
• Instructions for recommended storage and handling
• Technical datasheet including validated protocols and suggested dilutions

Recommended use notes: For optimal results, titrate antibody for each application and sample type. Use appropriate positive and negative controls to confirm specificity. For Western blotting, begin with manufacturer-recommended dilutions and optimize blocking and incubation conditions. For immunoprecipitation and flow cytometry, ensure compatible secondary reagents and buffers. Follow institutional guidelines for handling and disposal of biological reagents and consult the product datasheet for detailed protocol recommendations and safety information.